Lifshits LA, Breuer Y, Sova M, Gupta S, Kadosh D, Weinberg E, Hayouka Z, Bar DZ, Gal M. Nature-inspired peptide of MtDef4 C-terminus tail enables protein delivery in mammalian cells. Sci Rep. 2024 Feb 26;14(1):4604. doi: 10.1038/s41598-024-55274-4. PubMed PMID: 38409451; PubMed Central PMCID: PMC10897151.

Gruzdev N, Hacham Y, Haviv H, Stern I, Gabay M, Bloch I, Amir R, Gal M, Yadid I. Conversion of methionine biosynthesis in Escherichia coli from trans- to direct-sulfurylation enhances extracellular methionine levels. Microb Cell Fact. 2023 Aug 11;22(1):151. doi: 10.1186/s12934-023-02150-x. PubMed PMID: 37568230; PubMed Central PMCID: PMC10416483.

Ansbacher T, Tohar R, Cohen A, Cohen O, Levartovsky S, Arieli A, Matalon S, Bar DZ, Gal M, Weinberg E. A novel computationally engineered collagenase reduces the force required for tooth extraction in an ex-situ porcine jaw model. J Biol Eng. 2023 Jul 17;17(1):47. doi: 10.1186/s13036-023-00366-4. PubMed PMID: 37461028; PubMed Central PMCID: PMC10353231.

Pinkhasov I, Kabakov L, Nemcovsky CE, Weinreb M, Schlesinger P, Bender O, Gal M, Bar DZ, Weinberg E. Single-cell transcriptomic analysis of oral masticatory and lining mucosa-derived mesenchymal stromal cells. J Clin Periodontol. 2023 Jun;50(6):807-818. doi: 10.1111/jcpe.13799. Epub 2023 Mar 8. PubMed PMID: 36864739.

Lifshits LA, Rabin M, Tohar R, Netti F, Gabay M, Sova M, Bar DZ, Weinberg E, Adler-Abramovich L, Gal M. Enhancement of Collagen-I Levels in Human Gingival Fibroblasts by Small Molecule Activation of HIF-1α. J Agric Food Chem. 2023 May 24;71(20):7829-7835. doi: 10.1021/acs.jafc.2c09059. Epub 2023 May 3. PubMed PMID: 37132511; PubMed Central PMCID: PMC10214441.

Tubiana J, Adriana-Lifshits L, Nissan M, Gabay M, Sher I, Sova M, Wolfson HJ, Gal M. Funneling modulatory peptide design with generative models: Discovery and characterization of disruptors of calcineurin protein-protein interactions. PLoS Comput Biol. 2023 Feb;19(2):e1010874. doi: 10.1371/journal.pcbi.1010874. eCollection 2023 Feb. PubMed PMID: 36730443; PubMed Central PMCID: PMC9928118.

Alali H, Bloch I, Rapaport I, Rodrigues L, Sher I, Ansbacher T, Gal M. Application of In Silico Filtering and Isothermal Titration Calorimetry for the Discovery of Small Molecule Inhibitors of MDM2. Pharmaceuticals (Basel). 2022 Jun 16;15(6). doi: 10.3390/ph15060752. PubMed PMID: 35745671; PubMed Central PMCID: PMC9230431.

Tohar R, Alali H, Ansbacher T, Brosh T, Sher I, Gafni Y, Weinberg E, Gal M. Collagenase Administration into Periodontal Ligament Reduces the Forces Required for Tooth Extraction in an Ex situ Porcine Jaw Model. J Funct Biomater. 2022 Jun 8;13(2). doi: 10.3390/jfb13020076. PubMed PMID: 35735930; PubMed Central PMCID: PMC9225053.

Bloch I, Haviv H, Rapoport I, Cohen E, Shushan RSB, Dotan N, Sher I, Hacham Y, Amir R, Gal M. Discovery and characterization of small molecule inhibitors of cystathionine gamma-synthase with in planta activity. Plant Biotechnol J. 2021 Sep;19(9):1785-1797. doi: 10.1111/pbi.13591. Epub 2021 May 12. PubMed PMID: 33773037; PubMed Central PMCID: PMC8428831.

Rogawski R, Rogel A, Bloch I, Gal M, Horovitz A, London N, Sharon M. Intracellular Protein-Drug Interactions Probed by Direct Mass Spectrometry of Cell Lysates. Angew Chem Int Ed Engl. 2021 Sep 1;60(36):19637-19642. doi: 10.1002/anie.202104947. Epub 2021 Jul 20. PubMed PMID: 34101963; PubMed Central PMCID: PMC8457057.

Tohar R, Ansbacher T, Sher I, Afriat-Jurnou L, Weinberg E, Gal M. Screening Collagenase Activity in Bacterial Lysate for Directed Enzyme Applications. Int J Mol Sci. 2021 Aug 9;22(16). doi: 10.3390/ijms22168552. PubMed PMID: 34445258; PubMed Central PMCID: PMC8395246.

Sun Y, Harpazi B, Wijerathna-Yapa A, Merilo E, de Vries J, Michaeli D, Gal M, Cuming AC, Kollist H, Mosquna A. A ligand-independent origin of abscisic acid perception. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24892-24899. doi: 10.1073/pnas.1914480116. Epub 2019 Nov 19. PubMed PMID: 31744875; PubMed Central PMCID: PMC6900503.

Sterlin Y, Pri-Tal O, Zimran G, Park SY, Ben-Ari J, Kourelis J, Verstraeten I, Gal M, Cutler SR, Mosquna A. Optimized small-molecule pull-downs define MLBP1 as an acyl-lipid-binding protein. Plant J. 2019 Jun;98(5):928-941. doi: 10.1111/tpj.14272. Epub 2019 Mar 19. PubMed PMID: 30735592.

Brand Shwartz M, Assor M, Dotan N, Ratzon E, Cohen E, Ruimi N, Bloch I, Gal M, Yadid I. Inhibition of PD1:PD-L1 interaction by an E. coli-derived optimized PD1 variant. Biochem Biophys Res Commun. 2018 Nov 30;506(3):731-738. doi: 10.1016/j.bbrc.2018.10.147. Epub 2018 Oct 30. PubMed PMID: 30384998.

Dotan N, Gayder V, Bloch I, Gal M. An ELISA for the study of calcineurin-NFAT unstructured region interaction. Anal Biochem. 2018 May 15;549:66-71. doi: 10.1016/j.ab.2018.03.014. Epub 2018 Mar 16. PubMed PMID: 29555327.

Ratzon E, Bloch I, Nicola M, Cohen E, Ruimi N, Dotan N, Landau M, Gal M. A Small Molecule Inhibitor of Bruton's Tyrosine Kinase Involved in B-Cell Signaling. ACS Omega. 2017 Aug 31;2(8):4398-4410. doi: 10.1021/acsomega.7b00576. eCollection 2017 Aug 31. PubMed PMID: 31457731; PubMed Central PMCID: PMC6641755.

Gal M, Bloch I, Shechter N, Romanenko O, Shir OM. Efficient Isothermal Titration Calorimetry Technique Identifies Direct Interaction of Small Molecule Inhibitors with the Target Protein. Comb Chem High Throughput Screen. 2016;19(1):4-13. doi: 10.2174/1386207319666151203001529. PubMed PMID: 26632443.

Gal M, Frydman L. Multidimensional NMR spectroscopy in a single scan. Magn Reson Chem. 2015 Nov;53(11):971-85. doi: 10.1002/mrc.4271. Epub 2015 Aug 6. PubMed PMID: 26249041.

Takeuchi K, Sun ZY, Li S, Gal M, Wagner G. NMR resonance assignments of the catalytic domain of human serine/threonine phosphatase calcineurin in unligated and PVIVIT-peptide-bound states. Biomol NMR Assign. 2015 Apr;9(1):201-5. doi: 10.1007/s12104-014-9574-y. Epub 2014 Sep 11. PubMed PMID: 25209144; PubMed Central PMCID: PMC4352383.

Gal M, Li S, Luna RE, Takeuchi K, Wagner G. The LxVP and PxIxIT NFAT motifs bind jointly to overlapping epitopes on calcineurin's catalytic domain distant to the regulatory domain. Structure. 2014 Jul 8;22(7):1016-27. doi: 10.1016/j.str.2014.05.006. Epub 2014 Jun 19. PubMed PMID: 24954618; PubMed Central PMCID: PMC4102887.

Gal M, Edmonds KA, Milbradt AG, Takeuchi K, Wagner G. Speeding up direct (15)N detection: hCaN 2D NMR experiment. J Biomol NMR. 2011 Dec;51(4):497-504. doi: 10.1007/s10858-011-9580-7. Epub 2011 Oct 30. PubMed PMID: 22038648; PubMed Central PMCID: PMC3338130.

Takeuchi K, Gal M, Takahashi H, Shimada I, Wagner G. HNCA-TOCSY-CANH experiments with alternate (13)C- (12)C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment. J Biomol NMR. 2011 Jan;49(1):17-26. doi: 10.1007/s10858-010-9456-2. Epub 2010 Nov 26. PubMed PMID: 21110064; PubMed Central PMCID: PMC3072286.

Gal M, Zibzener K, Frydman L. A capacitively coupled temperature-jump arrangement for high-resolution biomolecular NMR. Magn Reson Chem. 2010 Nov;48(11):842-7. doi: 10.1002/mrc.2675. PubMed PMID: 20818777.

Lee MK, Gal M, Frydman L, Varani G. Real-time multidimensional NMR follows RNA folding with second resolution. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9192-7. doi: 10.1073/pnas.1001195107. Epub 2010 May 3. PubMed PMID: 20439766; PubMed Central PMCID: PMC2889053.

Gal M, Frydman L. Single-scan 2D NMR correlations by multiple coherence transfers. J Magn Reson. 2010 Apr;203(2):311-5. doi: 10.1016/j.jmr.2009.10.013. Epub 2009 Nov 3. PubMed PMID: 20149697.

Corazza A, Rennella E, Schanda P, Mimmi MC, Cutuil T, Raimondi S, Giorgetti S, Fogolari F, Viglino P, Frydman L, Gal M, Bellotti V, Brutscher B, Esposito G. Native-unlike long-lived intermediates along the folding pathway of the amyloidogenic protein beta2-microglobulin revealed by real-time two-dimensional NMR. J Biol Chem. 2010 Feb 19;285(8):5827-35. doi: 10.1074/jbc.M109.061168. Epub 2009 Dec 22. PubMed PMID: 20028983; PubMed Central PMCID: PMC2820808.

Gal M, Kern T, Schanda P, Frydman L, Brutscher B. An improved ultrafast 2D NMR experiment: towards atom-resolved real-time studies of protein kinetics at multi-Hz rates. J Biomol NMR. 2009 Jan;43(1):1-10. doi: 10.1007/s10858-008-9284-9. Epub 2008 Nov 4. PubMed PMID: 18982409.

Herrera A, Fernández-Valle E, Martínez-Alvarez R, Molero D, Pardo ZD, Sáez E, Gal M. Real-time monitoring of organic reactions with two-dimensional ultrafast TOCSY NMR spectroscopy. Angew Chem Int Ed Engl. 2009;48(34):6274-7. doi: 10.1002/anie.200902387. PubMed PMID: 19603443.

Mishkovsky M, Gal M, Frydman L. Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments. J Biomol NMR. 2007 Dec;39(4):291-301. doi: 10.1007/s10858-007-9195-1. Epub 2007 Oct 17. PubMed PMID: 17940728.

Gal M, Schanda P, Brutscher B, Frydman L. UltraSOFAST HMQC NMR and the repetitive acquisition of 2D protein spectra at Hz rates. J Am Chem Soc. 2007 Feb 7;129(5):1372-7. doi: 10.1021/ja066915g. PubMed PMID: 17263421.

NEW METHIONINE METABOLIC PATHWAY INHIBITORS (2019)

      I. Bloch, E. Cohen, R. Amir and M. Gal

M. Gal. Single scan NMR. Fast NMR data acquisition: Beyond the Fourier Transformation. Editors: Mehdi Mobi and Jeffery Hoch. 2017

K. Takeuchi, M. Gal, I. Shimada and G. Wagner. Low-gama detection in NMR spectroscopy. Recent Developments in Bio-molecular NMR. , Editors: Marius Clore and Jennifer Pott. 2012